Key result
S-nitrosothiol NO donors directly inhibit expressed cardiovascular L-type Ca2+ channels in HEK293 cells by a mechanism independent of cGMP.
Population
Human embryonic kidney cells expressing cardiovascular L-type Ca2+ channels
Design
Preclinical
Authors
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SNAP's cGMP-independent Ca channel block in HEK cells warrants no clinical action; leaves open relevance to native cardiomyocytes.
Effect estimate: EC50 of 360 μmol/L for α1C + β1a channels (SNAP)
S-nitrosothiol NO donors directly inhibit expressed cardiac L-type Ca2+ channels via a cGMP-independent mechanism.
Hai Hu (1997) studied Expressed cardiovascular L-type Ca2+ channels in HEK293 cells. S-nitrosothiol NO donors (SNAP, SNC, GSNO) was evaluated on Amplitude of barium currents (IBa) through L-type Ca2+ channels (EC50 of 360 μmol/L for α1C + β1a channels (SNAP)). S-nitrosothiol NO donors directly inhibit expressed cardiovascular L-type Ca2+ channels in HEK293 cells by a mechanism independent of cGMP.
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