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May 1, 1984Proceedings of the National Academy of SciencesOpen Access

Pulse labeling of small nuclear ribonucleoproteins in vivo reveals distinct patterns of antigen recognition by human autoimmune antibodies.

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Key result

Pulse-labeling of snRNPs revealed that Sm sera precipitate all eight snRNP proteins, whereas RNP antibodies initially precipitate only proteins A and C.

Population

Cells pulse-labeled with [35S]methionine and sera from patients with systemic lupus erythematosus and mixed…

Design

Preclinical

Authors

DFDavid E. FisherRutgers, The State University of New JerseyWRWestley H. ReevesUniversity of Florida HealthGCG E ConnerUniversity of Miami

Discussion

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Implication

Antigen specificity distinctions remain difficult to define; leaves open refined diagnostic separation of SLE versus MCTD.

Structured PICO

P
Population
In vitro study using human myeloid K562 cells and sera from over 20 patients with SLE or MCTD to analyze Sm and RNP antibody antigen recognition.
I
Intervention
Pulse-labeling with [35S]methionine for 8 min followed by immunoprecipitation
O
Outcome
Patterns of Sm and RNP immunorecognition of snRNP proteinssurrogate

Pulse-labeling of snRNPs reveals distinct patterns of antigen recognition by Sm and RNP antibodies, clarifying the biochemical basis of standard laboratory tests for these reactivities.

Limitations

  • The methionine-deficient protein discovered by Kinlaw et al. was not examined in this study.

Cite This Study

Fisher et al. (1984) studied Systemic lupus erythematosus (SLE) and mixed connective tissue disease (MCTD). Pulse labeling with [35S]methionine vs. Extended labeling was evaluated on Pattern of snRNP protein immunoprecipitation by Sm and RNP sera. Pulse-labeling of snRNPs revealed that Sm sera precipitate all eight snRNP proteins, whereas RNP antibodies initially precipitate only proteins A and C.

synapsesocial.com/papers/6a6e12e3ce524a4339c2e23dhttps://doi.org/10.1073/pnas.81.10.3185
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Protein antigens of the RNA-protein complexes detected by anti-SM and anti-RNP antibodies found in serum of patients with systemic lupus erythematosus and related disorders.1982 · 73 citations
  2. 2A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels1974 · 8,644 citations
  3. 3Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus2001 · 550 citations
  4. 4Physical Association of Two Nuclear Antigens and Mutual Occurrence of Their Antibodies: The Relationship of the Sm and Rnaprotein (MO) Systems in Sle Sera1973 · 146 citations
  5. 5Characterization of a Soluble Nuclear Ribonucleoprotein Antigen Reactive with Sle Sera1971 · 217 citations