Key result
An altered nonstructural protein, 3A, is a primary determinant of restricted growth of the Taiwanese foot-and-mouth disease virus on bovine cells and contributes to its attenuation in bovines.
Population
In vitro (BHK and BK cells) and in vivo (bovines) models
Comparison
Genetically engineered chimeric viruses created… vs Wild-type or other chimeric FMD viruses
Design
Preclinical
Follow-up
3 weeks
Authors
Loading...
Highlights 3A as a vaccine attenuation target in bovines; leaves open translation from in vitro data to in vivo efficacy.
The 3A nonstructural protein is the primary genetic determinant for the restricted bovine virulence of the Taiwanese foot-and-mouth disease virus.
Beard et al. (2000) studied Foot-and-mouth disease (FMD). Genetically engineered chimeric FMD viruses (OTai 3A substitution) vs. Wild-type or other chimeric FMD viruses was evaluated on Viral growth on bovine cells in vitro and virulence in bovines in vivo. An altered nonstructural protein, 3A, is a primary determinant of restricted growth of the Taiwanese foot-and-mouth disease virus on bovine cells and contributes to its attenuation in bovines.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: