An altered nonstructural protein, 3A, is a primary determinant of restricted growth of the Taiwanese foot-and-mouth disease virus on bovine cells and contributes to its attenuation in bovines.
The 3A nonstructural protein is the primary genetic determinant for the restricted bovine virulence of the Taiwanese foot-and-mouth disease virus.
In 1997, a devastating outbreak of foot-and-mouth disease (FMD) in Taiwan was caused by a serotype O virus (referred to here as OTai) with atypical virulence. It produced high morbidity and mortality in swine but did not affect cattle. We have defined the genetic basis of the species specificity of OTai by evaluating the properties of genetically engineered chimeric viruses created from OTai and a bovine-virulent FMD virus. These studies have shown that an altered nonstructural protein, 3A, is a primary determinant of restricted growth on bovine cells in vitro and significantly contributes to bovine attenuation of OTai in vivo.
Beard et al. (Sat,) conducted a other in Foot-and-mouth disease (FMD). Genetically engineered chimeric FMD viruses (OTai 3A substitution) vs. Wild-type or other chimeric FMD viruses was evaluated on Viral growth on bovine cells in vitro and virulence in bovines in vivo. An altered nonstructural protein, 3A, is a primary determinant of restricted growth of the Taiwanese foot-and-mouth disease virus on bovine cells and contributes to its attenuation in bovines.