Key result
A high-fat diet induced obesity in rats, leading to higher renal endothelin-1 expression which strongly correlated with increased glomerular mesangial expansion.
Why the study?
The study aimed to determine ET-1 expression levels in serum and renal tissue associated with changes in kidney structure.
Local renal ET-1 expression, rather than serum levels, correlates with glomerular impairment in a high-fat diet rat model.
Answers are grounded in this paper and cite their sources.
ET-1 associates with glomerular changes in obese rats; hypothesis-generating for human obesity-related nephropathy.
Objective: This study aimed to determinelevels of expression ET-1 in serum and renal tissue associated withchanges in kidney structure.Method: The rats were divided into two groups. The Obese group was given a high-fat-induced diet chowand a standard diet chow for the control group. ET-1 protein expression and localization in the glomerulusof the kidney were examined. The serum of blood checked for ET-Iusing ELISA method and assessed theET-1 expression of the glomeruliby Immunohistochemistry (IHC). Mesangial expansion is an indicator toevaluate the degree of glomerular damage by Periodic Acid–Schiff(PAS staining). Associations between theimmunoreactivity for ET-1 in glomeruli and the degree of kidney injury were examined.Results: Lee Index was significantly higher in the obese group than in the control group (p<0.05). ET-1immunoreactivity was considerably higher in the obese group than the control rat. It was a strong correlationwith enhancingmesangial expansion glomeruli using PAS staining of the renal tissue (rs=0.765; p= 0.001).Contrast to ET-1 levels in the serum revealed no significant in obese rat groups (p>0.05).Conclusion: These data confirm the role of ET-1 will provide changes in the levels of glomeruli impairment,but serum levels may well fail to reflect the local expression at the tissue level.
No takes yet. Share an insight, caveat, or question.
Haslindah Dahlan (2020) studied Obesity-induced glomerular impairment (n=16). High-fat diet vs. Standard laboratory diet was evaluated on Correlation between glomerular ET-1 expression and mesangial expansion (rs=0.765, p=0.001). A high-fat diet induced obesity in rats, leading to higher renal endothelin-1 expression which strongly correlated with increased glomerular mesangial expansion.
Synapse has enriched 10 related papers. Consider them for comparative context: