A blastogenic test using microtitre plates can detect peripheral blood leukocytes sensitized to foot-and-mouth disease virus antigen.
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Optimizes microtitre blastogenic assay conditions for FMDV; leaves open translation to veterinary diagnostics pending further validation.
A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 x 10(5) cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2-3 days.
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Wardley et al. (1979) studied this question.
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