What question did this study set out to answer?

To develop and validate an in vitro method using surviving bovine tongue epithelial tissue for titrating foot-and-mouth disease virus.

March 1, 1954Journal of HygieneOpen Access

Titration of the virus of foot-and-mouth disease in culture

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To develop and validate an in vitro method using surviving bovine tongue epithelial tissue for titrating foot-and-mouth disease virus.
Cultured surviving epithelial tissue from cattle tongues in cups on Perspex plates and detected viral multiplication using a complement-fixation test.
Compared the sensitivity of in vitro culture titration against standard intradermal tongue inoculation in live cattle and evaluated its use in antibody neutralization tests.
Demonstrated qualitative sensitivity equivalent to intradermal cattle tongue inoculation for the detection and titration of foot-and-mouth disease virus.
Established successful application of the tissue culture technique for detecting specific antibodies in neutralization tests.

Structured PICO

Population

Surviving epithelial tissue from the tongues of cattle

Intervention

Titration of the virus of foot-and-mouth disease in culture (incubated in cups on Perspex plates, detection by complement-fixation test)

Comparator

Titration by intradermal inoculation of the tongue of cattle

Outcome

Sensitivity for the detection of virussurrogate

A culture-based titration method for foot-and-mouth disease virus using cattle tongue epithelial tissue is as sensitive as in vivo intradermal inoculation.

Discussion

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Implication

May enable in vitro FMDV titration without live cattle; leaves open standardization for routine diagnostic or research use.

Cite This Study

Brooksby et al. (1954) studied this question.

synapsesocial.com/papers/6a8327dc162ea9d73e990a55 https://doi.org/10.1017/s0022172400027285

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Titration of the virus of foot-and-mouth disease in culture

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Titration of the virus of foot-and-mouth disease in culture

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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