Optimizing pH control and aeration in a stirred culture system significantly increases the yield of foot-and-mouth disease virus from bovine tongue epithelium explants.
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Refines explant culture systems for FMDV; extends in vitro methods but leaves open translation to vaccines or diagnostics.
A simple, laboratory scale, stirred system has been developed to examine conditions of culture necessary for optimum return of infective virus from explants of surviving bovine tongue epithelium, with particular bias towards large-scale procedures. Control of hydrogen-ion concentration and aeration of the culture are emphasized. The authors wish to thank Dr I. A. Galloway and Dr J. B. Brooksby for their helpful advice in preparing this paper, Mrs J. Lansley for her untiring technical assistance and Mr J. Goodridge who constructed the culture apparatus.
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Morrow et al. (1964) studied this question.
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