Key result
The NAC-ELISA demonstrated 100% sensitivity and 99.04% specificity for detecting neutralizing antibodies against FMDV serotype A, and strongly correlated with the virus neutralization test (r = 0.9334).
Why the study?
The virus neutralization test requires high-containment facilities capable of handling live viruses and is unsuitable for large-scale serological surveillance.
Effect estimate: r = 0.9334
p-value: p=<0.0001
A novel competitive ELISA using bovine monoclonal antibodies was developed as a potential alternative to the gold standard VNT for detecting FMDV neutralizing antibodies without requiring high-containment facilities.
Advances bovine bnAb tech for FMDV; leaves open whether derived ELISAs can replace VNT in practice.
The level of neutralizing antibodies in vaccinated animals is directly related to their level of protection against a virus challenge. The virus neutralization test (VNT) is a “gold standard” method for detecting neutralizing antibodies against foot-and-mouth disease virus (FMDV). However, VNT requires high-containment facilities that can handle live viruses and is not suitable for large-scale serological surveillance. In this study, a bovine broadly neutralizing monoclonal antibody (W145) against FMDV serotype A was successfully produced using fluorescence-based single-B-cell antibody technology.
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Cao et al. (2022) studied Foot-and-mouth disease virus (FMDV) serotype A (n=780). NAC-ELISA vs. Virus neutralization test (VNT) and LPB-ELISA was evaluated on Correlation between NAC-ELISA titers and VNT titers (r = 0.9334, p=<0.0001). The NAC-ELISA demonstrated 100% sensitivity and 99.04% specificity for detecting neutralizing antibodies against FMDV serotype A, and strongly correlated with the virus neutralization test (r = 0.9334).
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