April 1, 1997Journal of VirologyOpen Access

Characterization of synthetic foot-and-mouth disease virus provirions separates acid-mediated disassembly from infectivity

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Population

BHK cells transfected with synthetic foot-and-mouth disease virus (FMDV) RNAs

Comparison

Mutations at Ala-85 and Asp-86 of VP0 vs Wild-type (WT) virus

Design

Preclinical

Authors

TKT KnipePlum Island Animal Disease Center ERElizabeth RiederNational Agricultural Statistics Service BBBarry BaxtAgricultural Research Service

Discussion

Member takes

Implication

VP0 cleavage is required for FMDV infectivity; leaves open its precise role in endosomal genome release for further study.

Structured PICO

Population

BHK cells transfected with synthetic foot-and-mouth disease virus (FMDV) RNAs

Intervention

Mutations at Ala-85 (A85) and Asp-86 (D86) of VP0 (A85N, A85H, D86N, A85F/D86K, D86C)

Comparator

Wild-type (WT) virus

Outcome

Infectivity, particle formation, acid sensitivity, and hydrophobicity of breakdown productssurrogate

Cleavage of the VP0 protein in FMDV is essential for infectivity and likely plays a role in the release of the viral genome from the endosomal compartment.

Cite This Study

Knipe et al. (1997) studied this question.

synapsesocial.com/papers/6a6f5703f44fa9f079dcc3a2 https://doi.org/10.1128/jvi.71.4.2851-2856.1997

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