Key Points
- To assess whether HOE 694, a specific Na+/H+ exchange inhibitor, reduces myocardial infarct size when administered before coronary occlusion or prior to reperfusion in pigs.
- Pentobarbitone-anaesthetized, open-chest pigs were subjected to 60 minutes of left anterior descending coronary artery (LADCA) occlusion followed by 2 hours of reperfusion.
- Pigs were assigned to three groups: pre-occlusion treatment (pre-TCO, n=7; HOE 694 7 mg/kg/15 min infused 15 min before occlusion), pre-reperfusion treatment (pre-REP, n=7; HOE 694 infused from 45 to 60 min of occlusion), or vehicle control (n=7).
- Myocardial infarct size was quantified as a percentage of the left ventricular risk region using the p-nitroblue tetrazolium method.
- Infarct size was significantly reduced to 4.1% (1.4%) in the pre-TCO group (P < 0.01) and to 38.2% (5.8%) in the pre-REP group (P < 0.05) compared to 77.7% (4.0%) in the control group.
- Reduction in infarct size was significantly greater when HOE 694 was administered before ischaemia compared to administration before reperfusion (P < 0.05).
Structured PICO
Does HOE 694 reduce infarct size in a swine model of myocardial ischaemia-reperfusion?
PPopulation21 pentobarbitone-anaesthetized, open-chest pigs subjected to a 60 min occlusion of the left anterior descending coronary artery (LADCA) followed by 2 h of reperfusion.
IInterventionHOE 694 (Na+/H+ exchange inhibitor) infusion (7 mg/kg/15 min) administered either 15 min before occlusion (pre-TCO group) or starting at 45 min of occlusion (pre-REP group).
OOutcomeInfarct size (as % of the left ventricular risk region) determined by the p-nitroblue tetrazolium method.surrogate
Inhibition of Na+/H+ exchange with HOE 694 significantly reduces infarct size in a swine model of ischaemia-reperfusion, especially when administered prior to ischaemia.