Thrombin regulation of platelet interaction with damaged vessel wall and isolated collagen type I at arterial flow conditions in a porcine model: effects of hirudins, heparin, and calcium chelation
Why the study?
Does thrombin inhibition with hirudin reduce platelet deposition on damaged vessel walls compared to heparin in a porcine model under arterial flow conditions?
Population
Porcine model evaluating platelet vessel wall interaction and thrombus growth on two biologic vascular…
Comparison
Natural hirudin and recombinant hirudin vs Heparinized blood and Ca-chelated blood
Design
Preclinical
Follow-up
5 minutes
Authors
Discussion
Loading...
Member takes
Implication
May support thrombin inhibition over heparin for reducing platelet deposition in arterial injury; leaves open translation from this porcine study.
Structured PICO
Does thrombin inhibition with hirudin reduce platelet deposition on damaged vessel walls compared to heparin in a porcine model under arterial flow conditions?
P
Population
Porcine model evaluating platelet vessel wall interaction and thrombus growth on two biologic vascular surfaces and one immobilized vessel wall component (digested collagen type I) under arterial flow conditions (shear rates 212 to 1,690/s)
I
Intervention
Natural hirudin and recombinant hirudin (r-hirudin) (e.g., 100 U/mL r-hirudin)
C
Comparator
Heparinized blood (1.8 +/- 0.2 U/mL) and Ca(2+)-chelated (citrated) blood
O
Outcome
Platelet deposition measured by In-111-labeled platelets after 5 minutes of perfusionsurrogate
Local thrombin generation at sites of severe vascular injury induces platelet activation and deposition despite therapeutic heparin levels, which can be reduced by specific thrombin inhibitors like hirudin.
Cite This Study
Badimón et al. (1991) studied this question.