Key result
In vitro, both heparin and hirudin inhibited thrombin binding to the rabbit aorta endothelium (IC50 0.1 U/ml for both), but only heparin inhibited binding to the exposed subendothelium (IC50 1.8 U/ml).
Why the study?
Does heparin or hirudin inhibit thrombin binding to normal and de-endothelialized rabbit aorta in vitro?
Population
Freshly-excised rabbit aorta wall (normal and de-endothelialized segments) in vitro
Comparison
Heparin or hirudin vs Each other (heparin vs hirudin)
Design
Preclinical
Authors
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Supports distinct endothelial versus subendothelial thrombin binding in vitro; hypothesis-generating for antithrombotic mechanisms, requires in vivo confirmation.
Does heparin or hirudin inhibit thrombin binding to normal and de-endothelialized rabbit aorta in vitro?
Heparin, but not hirudin, inhibits thrombin binding to the exposed subendothelium in rabbit aortas, suggesting different binding site mechanisms.
Hatton et al. (1991) studied this question. Heparin vs. Hirudin was evaluated on Inhibition of 125I-thrombin binding to the endothelium and subendothelium. In vitro, both heparin and hirudin inhibited thrombin binding to the rabbit aorta endothelium (IC50 0.1 U/ml for both), but only heparin inhibited binding to the exposed subendothelium (IC50 1.8 U/ml).
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