March 21, 2013AJP Renal PhysiologyOpen Access

Dose-dependent effects of angiotensin-(1–7) on the NHE3 exchanger and Ca2+iin in vivo proximal tubules

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Why the study?

Does angiotensin-(1-7) alter bicarbonate reabsorption and intracellular calcium concentration in in vivo proximal tubules of rats?

Population

Male Wistar rats (220-350 g for in vivo micropuncture, 80 g for isolated proximal tubules)

Comparison

Angiotensin- [ANG-] at doses of 10^-12, 10^-9… vs Intratubular perfusion control solution alone

Design

Preclinical

Follow-up

Acute (minutes)

Authors

RCRegiane Cardoso Castelo‐BrancoHospital Universitario Fundación Jiménez Díaz DLDeise C. A. Leite-DelovaUniversidade de São Paulo MMM. Mello-AiresUniversidade de São Paulo

Discussion

Member takes

Implication

Should not yet change practice; hypothesis-generating for Mas receptor modulation of proximal tubule bicarbonate reabsorption.

Structured PICO

Does angiotensin-(1-7) alter bicarbonate reabsorption and intracellular calcium concentration in in vivo proximal tubules of rats?

Population

Male Wistar rats (220-350 g for in vivo micropuncture, 80 g for isolated proximal tubules)

Intervention

Angiotensin-(1-7) [ANG-(1-7)] at doses of 10^-12, 10^-9, or 10^-6 M via luminal microperfusion, alone or with A779, S3226, BAPTA, or thapsigargin

Comparator

Intratubular perfusion control solution alone

Outcome

Net bicarbonate reabsorption (JHCO3-) and intracellular Ca2+ concentration ([Ca2+]i)surrogate

Angiotensin-(1-7) exerts a biphasic, dose-dependent effect on bicarbonate reabsorption in the proximal tubule mediated by Mas receptors via the NHE3 exchanger, associated with transient increases in intracellular calcium.

Limitations

Whether [Ca2+]i modification is an important direct mechanism for NHE3 activation by these peptides or is a side effect of other signaling pathways will require additional studies.

Cite This Study

Castelo‐Branco et al. (2013) studied this question.

synapsesocial.com/papers/6a6f6a45f44fa9f079dcd9b4 https://doi.org/10.1152/ajprenal.00401.2012

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Dose-dependent effects of angiotensin-(1–7) on the NHE3 exchanger and Ca2+iin in vivo proximal tubules

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Dose-dependent effects of angiotensin-(1–7) on the NHE3 exchanger and Ca2+iin in vivo proximal tubules

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider