Key result
In MDCK cells, Angiotensin II stimulated or impaired intracellular pH recovery depending on concentration, an effect that was impaired by ANP or calcium chelation.
Why the study?
Does the interaction of ANG II and ANP affect intracellular pH recovery and cytosolic free calcium concentration in MDCK cells?
Population
Madin-Darby canine kidney (MDCK) cells
Comparison
Angiotensin II and atrial natriuretic peptide vs Control situation
Design
Preclinical
Authors
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No immediate clinical implications for renal pH or hypertension management; leaves open in vivo and human translation of these cell-level interactions.
Does the interaction of ANG II and ANP affect intracellular pH recovery and cytosolic free calcium concentration in MDCK cells?
The study demonstrates that intracellular calcium regulates the Na+/H+ exchanger-mediated pH recovery in MDCK cells, which is modulated by the interaction between angiotensin II and atrial natriuretic peptide.
Oliveira‐Souza et al. (2000) studied this question. Angiotensin II and atrial natriuretic peptide vs. Control was evaluated on Intracellular pH recovery rate and cytosolic free calcium concentration. In MDCK cells, Angiotensin II stimulated or impaired intracellular pH recovery depending on concentration, an effect that was impaired by ANP or calcium chelation.