What question did this study set out to answer?

This research investigates how angiotensin II affects Na+-independent Cl--HCO3- exchange in cardiac muscle.

March 9, 1998Circulation ResearchOpen Access

Angiotensin II Activates Na + -Independent Cl − -HCO 3 − Exchange in Ventricular Myocardium

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Population

Cat papillary muscles

Comparison

Angiotensin II (500 nmol/L) vs Absence of Angiotensin II or presence of…

Design

Preclinical

Authors

MHMarı́a C. Camilión de HurtadoBoehringer Ingelheim (Germany)BÁBernardo V. ÁlvarezConsejo Nacional de Investigaciones Científicas y Técnicas NPNéstor G. PérezApple (Israel)

Discussion

Member takes

Implication

May modulate myocardial pH recovery in feline models via AT1-PKC; leaves open translation to human ventricular pathophysiology.

Key Points

This research investigates how angiotensin II affects Na+-independent Cl--HCO3- exchange in cardiac muscle.
Measured intracellular pH (pHi) in cat papillary muscles using epifluorescence and BCECF-AM.
Evaluated the effects of angiotensin II (500 nmol/L) on pHi recovery in different buffer conditions with inhibitors for validation.
Used specific inhibitors like SITS and receptor antagonists to confirm pathways involved.
Ang II increased pHi from 7.05+/-0.05 to 7.22+/-0.05 in Na+-free, bicarbonate-buffered conditions (P<.05).
The rate of pHi recovery was doubled in the presence of Ang II, indicating AE stimulation (0.053+/-0.016 to 0.108+/-0.026 pH unit/min, P<.05).
Effects were blocked by AT1 receptor antagonist losartan and protein kinase C inhibitors, suggesting a specific regulatory pathway.

Structured PICO

Population

Cat papillary muscles

Intervention

Angiotensin II (500 nmol/L)

Comparator

Absence of Angiotensin II or presence of specific inhibitors (SITS, S20787, losartan, chelerythrine, calphostin C)

Outcome

Intracellular pH (pHi) and velocity of myocardial pHi recoverysurrogate

Angiotensin II activates the Na+-independent Cl--HCO3- exchanger in ventricular myocardium via a protein kinase C-dependent pathway linked to AT1 receptors.

Cite This Study

Hurtado et al. (1998) studied this question.

synapsesocial.com/papers/6a70c7e3febe604dd7098c2a https://doi.org/10.1161/01.res.82.4.473

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