What question did this study set out to answer?

September 1, 1984Journal of NeuroscienceOpen Access

Immunocytochemical localization of the mammalian voltage-dependent sodium channel using polyclonal antibodies against the purified protein

View Full Paper

Ask AI

Bookmark

Authors

BHBeatrice HaimovichRutgers, The State University of New Jersey EBEdmundo BonillaUniversidad Autónoma Metropolitana JCJan CasadeiNational Institutes of Health

Discussion

Member takes

Implication

Demonstrates the immunocytochemical localization of sodium channels in mammalian muscle and nerve tissues, suggesting shared antigenic markers across species.

Key Points

To establish the localization of the voltage-dependent sodium channel using polyclonal antibodies raised against purified protein.
Rabbits immunized with purified sodium channel protein from rat skeletal muscle.
Antiserum tested through radioimmunoassays and immunoblots of membrane proteins.
Localization in tissues confirmed via immunocytochemical techniques and chromatography methods.
Antiserum specifically bound to the surface membranes of rat skeletal muscle, cardiac muscle, and peripheral nerve.
Cross-reactivity observed in mouse, human, and guinea pig skeletal muscle but not in chicken, rabbit, or frog muscle.
Results indicate common antigenic determinants in sodium channels of various mammalian muscle and nerve tissues.

Cite This Study

Haimovich et al. (1984) studied this question.

synapsesocial.com/papers/6a6f763ce71d69abee0807fc https://doi.org/10.1523/jneurosci.04-09-02259.1984

View Full Paper

Ask AI

Bookmark

Implication

Demonstrates the immunocytochemical localization of sodium channels in mammalian muscle and nerve tissues, suggesting shared antigenic markers across species.

Immunocytochemical localization of the mammalian voltage-dependent sodium channel using polyclonal antibodies against the purified protein

Discussion

Member takes

Implication

Key Points

Cite This Study

Immunocytochemical localization of the mammalian voltage-dependent sodium channel using polyclonal antibodies against the purified protein

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider