What question did this study set out to answer?

Characterize the polypeptide subunits of voltage-sensitive calcium channel dihydropyridine receptors and assess their conservation across multiple tissues and animal species.

February 9, 1987FEBS LettersOpen Access

Monoclonal antibodies against the 1,4‐dihydropyridine receptor associated with voltage‐sensitive Ca2+ channels detect similar polypeptides from a variety of tissues and species

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Monoclonal antibodies against voltage-sensitive Ca2+ channel dihydropyridine receptors. Primary outcome: Detection of polypeptides by immunoblot assay.

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Key result

Four monoclonal antibodies against voltage-sensitive Ca2+ channel dihydropyridine receptors recognized a single polypeptide of Mr ~140,000 in reducing gels across various tissues and species.

Population

Rabbit skeletal muscle, heart, and brain tissues, and skeletal muscle of other species

Design

Preclinical

Authors

RNRobert I. NormanUniversity of Leicester ABAlison J. BurgessUniversity of Southampton EAEmily AllenGonzaga University

Discussion

Member takes

Implication

Antibodies reveal conserved Ca2+ channel polypeptides across tissues and species; leaves open functional equivalence and therapeutic relevance in humans.

Key Points

Characterize the polypeptide subunits of voltage-sensitive calcium channel dihydropyridine receptors and assess their conservation across multiple tissues and animal species.
Generated four monoclonal antibodies against dihydropyridine receptors purified from rabbit skeletal muscle.
Conducted immunoblot assays on denatured transverse tubule membranes separated via reducing and nonreducing polyacrylamide gel electrophoresis across rabbit muscle, heart, brain, and other species.
Under reducing conditions, all four antibodies recognized a single ~140,000 Mr polypeptide co-migrating with the purified receptor's large glycoprotein subunit.
Under nonreducing conditions, antibodies bound to an ~170,000 Mr protein, with three antibodies also binding larger complexes at ~310,000 and ~330,000 Mr.
Cross-reactive polypeptides matching these molecular masses were identified in rabbit heart, rabbit brain, and skeletal muscle tissue from other species.

Structured PICO

Population

Rabbit skeletal muscle, heart, and brain tissues, and skeletal muscle of other species

Intervention

Four monoclonal antibodies against voltage-sensitive Ca2+ channel dihydropyridine receptors

Outcome

Detection of polypeptides by immunoblot assaysurrogate

Monoclonal antibodies against rabbit skeletal muscle Ca2+ channel dihydropyridine receptors cross-react with similar polypeptides in heart, brain, and other species, suggesting conserved structural components.

Cite This Study

Norman et al. (1987) studied this question. Monoclonal antibodies against voltage-sensitive Ca2+ channel dihydropyridine receptors was evaluated on Detection of polypeptides by immunoblot assay. Four monoclonal antibodies against voltage-sensitive Ca2+ channel dihydropyridine receptors recognized a single polypeptide of Mr ~140,000 in reducing gels across various tissues and species.

synapsesocial.com/papers/6a8df315943ed3bfed09cfd5 https://doi.org/10.1016/0014-5793(87)81570-3

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Implication

Antibodies reveal conserved Ca2+ channel polypeptides across tissues and species; leaves open functional equivalence and therapeutic relevance in humans.

Monoclonal antibodies against the 1,4‐dihydropyridine receptor associated with voltage‐sensitive Ca2+ channels detect similar polypeptides from a variety of tissues and species

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Monoclonal antibodies against the 1,4‐dihydropyridine receptor associated with voltage‐sensitive Ca2+ channels detect similar polypeptides from a variety of tissues and species

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider