What question did this study set out to answer?

This research examines how voltage-activated calcium channels respond to membrane depolarization and the role of phosphorylation in this process.

Voltage-activated calcium channels that must be phosphorylated to respond to membrane depolarization.

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Randomized trial uncovers the need for phosphorylation of calcium channels for activation in pituitary cells, suggesting new pathways for modulation.

This research examines how voltage-activated calcium channels respond to membrane depolarization and the role of phosphorylation in this process.
Activated calcium channels were analyzed in cell-free membrane patches from GH3 cells.
Calcium concentration and phosphorylation conditions were manipulated to observe channel responses.
The effects of dihydropyridine BAY K 8644 on channel activity were assessed.
One class of calcium channels had a conductance of approximately 10 pS with rapid inactivation, while another class exhibited 23 pS conductance and did not inactivate in barium.
Phosphorylation with cAMP-dependent protein kinase and MgATP restored channel activity after loss.
BAY K 8644 delayed but could not restore calcium channel activity once lost.

Armstrong et al. (1987) studied this question.

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Randomized trial uncovers the need for phosphorylation of calcium channels for activation in pituitary cells, suggesting new pathways for modulation.