Identification of group A rotavirus gene 4 types by polymerase chain reaction
Population
16 well-characterized human rotavirus isolates and HRV isolates from stool samples of patients with diarrhea
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May aid rotavirus epidemiologic research; leaves open clinical adoption pending human studies.
Structured PICO
P
Population
16 well-characterized human rotavirus (HRV) isolates and HRV isolates from stool samples of patients with diarrhea
I
Intervention
Polymerase chain reaction (PCR) typing method using a cocktail of conserved and type-specific primers
O
Outcome
Identification of HRV gene 4 types (1, 2, 3, and 4)surrogate
A novel PCR-based typing method allows for direct and reliable identification of human rotavirus gene 4 types from stool samples, aiding epidemiologic and vaccine research.
Cite This Study
Gentsch et al. (1992) studied this question.