Key result
A seminested PCR typing assay identified the P[14] genotype in 1 of 4 human rotavirus strains with unusual subgroup-electropherotype linkage and 0 of 50 strains with usual linkage.
A seminested PCR typing assay can successfully identify human rotavirus strains with the P[14] genotype.
Seminested PCR may detect rare P[14] rotavirus in atypical strains; leaves open clinical adoption pending larger human validation.
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
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Arista et al. (1999) studied this question. A seminested PCR typing assay identified the P[14] genotype in 1 of 4 human rotavirus strains with unusual subgroup-electropherotype linkage and 0 of 50 strains with usual linkage.
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