Key result
In a survey of 199 human rotavirus strains from infants in Japan and Thailand, the P1A gene 4 allele was the most prevalent, detected in 165 of the samples.
Cross-Sectional (n=199)
Yes
PCR is a highly specific and sensitive proxy method to predict rotavirus P serotype, revealing that the P1A gene 4 allele is the most prevalent in the surveyed regions of Japan and Thailand.
Supports regional rotavirus genotyping; leaves open implications for vaccine strain selection.
The presence of six gene 4 alleles (or VP4 genotypes) in human rotaviruses has been recognized. Using 16 representative cultivable human rotavirus strains, we confirmed the specificity of VP4 genotyping by polymerase chain reaction (PCR) using the nested oligonucleotides specific to each of the four representative gene 4 alleles. Using the PCR, we surveyed the gene 4 alleles of 199 human rotaviruses in stools collected in Japan and Thailand. Strains with the gene 4 allele, corresponding to P1A serotype, were shown to be the most prevalent, but two strains with P2 gene 4 allele and one strain with P3 gene 4 allele were detected in Thailand and in Japan, respectively.
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Wu et al. (1994) conducted a cross-sectional in Rotavirus gastroenteritis (n=199). Gene 4 allele distribution was evaluated on Prevalence of gene 4 alleles (VP4 genotypes). In a survey of 199 human rotavirus strains from infants in Japan and Thailand, the P1A gene 4 allele was the most prevalent, detected in 165 of the samples.
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