October 1, 1984Journal of Biological ChemistryOpen Access

Oligomeric regulation of the later reaction steps of the sarcoplasmic reticulum calcium ATPase.

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Population

Sarcoplasmic reticulum Ca2+ ATPase (in vitro model)

Comparison

Chasing with EGTA or excess [gamma-31P]ATP… vs Different phases of E32P formation or different…

Design

Preclinical

Authors

NINoriaki IkemotoDenso (Japan)RNRandall W. NelsonPhoenix (United States)

Discussion

Member takes

Implication

Advances SERCA oligomer model in vitro; leaves open in vivo relevance to cardiac Ca2+ handling.

Structured PICO

Population

Sarcoplasmic reticulum Ca2+ ATPase (in vitro model)

Intervention

Chasing with EGTA or excess [gamma-31P]ATP; addition of nonionic detergent ethylene glycol monoether or cross-linker dithiobissuccinimidyl propionate

Comparator

Different phases of E32P formation or different chasing conditions

Outcome

Kinetics of phosphorylated intermediate (EP) decompositionsurrogate

The study provides biochemical evidence that the sarcoplasmic reticulum Ca2+ ATPase functions as an oligomer with sequential reaction steps among its subunits.

Cite This Study

Ikemoto et al. (1984) studied this question.

synapsesocial.com/papers/6a6ff94cfebe604dd708c9de https://doi.org/10.1016/s0021-9258(20)71281-0

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Oligomeric regulation of the later reaction steps of the sarcoplasmic reticulum calcium ATPase.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Oligomeric regulation of the later reaction steps of the sarcoplasmic reticulum calcium ATPase.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider