April 1, 1983Journal of Biological ChemistryOpen Access

Reversible control of oligomeric interaction of the sarcoplasmic reticulum calcium ATPase with the use of a cleavable cross-linking agent.

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Population

Fragmented sarcoplasmic reticulum (Ca2+ ATPase polypeptides)

Comparison

Reversible cross-linking with a cleavable… vs Different conditions

Design

Preclinical

Authors

YKYoshiko KurobeOsaka Medical and Pharmaceutical University RNRandall W. NelsonPhoenix (United States)NINoriaki IkemotoDenso (Japan)

Discussion

Member takes

Implication

Does not guide clinical decisions; leaves open whether SERCA subunit interactions can be therapeutically targeted in human cardiac disease.

Structured PICO

Population

Fragmented sarcoplasmic reticulum (Ca2+ ATPase polypeptides)

Intervention

Reversible cross-linking with a cleavable homobifunctional reagent, dithiobissuccinimidyl propionate

Comparator

Different conditions (presence/absence of ATP, high/low Ca2+, cleaved/uncleaved cross-links)

Outcome

Ca2+-induced conformational changes, phosphoenzyme (EP) formation, presteady state Ca2+ translocation, and EP decompositionsurrogate

Reversible cross-linking of sarcoplasmic reticulum Ca2+ ATPase demonstrates that extensive subunit interaction is required for Ca2+-induced conformational changes.

Cite This Study

Kurobe et al. (1983) studied this question.

synapsesocial.com/papers/6a6ff9bcf44fa9f079dd7c82 https://doi.org/10.1016/s0021-9258(18)32634-6

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Implication

Does not guide clinical decisions; leaves open whether SERCA subunit interactions can be therapeutically targeted in human cardiac disease.

Reversible control of oligomeric interaction of the sarcoplasmic reticulum calcium ATPase with the use of a cleavable cross-linking agent.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Reversible control of oligomeric interaction of the sarcoplasmic reticulum calcium ATPase with the use of a cleavable cross-linking agent.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider