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November 1, 1979Journal of Biological ChemistryOpen Access

Irreversible thiophosphorylation and activation of tension in functionally skinned rabbit ileum strips by 35SATP gamma S.

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Population

Rabbit ileum strips functionally skinned by exposure to staphylococcal alpha-toxin

Comparison

Incubation in ATP analog ATP gamma S or [35S]ATP… vs Incubation in the absence of Ca2+, or exposure…

Design

Preclinical

Authors

PCPaul CassidyTrinity College DublinPHPhyllis E. HoarUniversity of MiamiWKW. Gleen L. KerrickUniversity of Miami

Discussion

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Implication

Supports Ca2+-sensitization mechanisms in smooth muscle; hypothesis-generating and requires human validation before any clinical consideration.

Structured PICO

P
Population
Rabbit ileum strips functionally skinned by exposure to staphylococcal alpha-toxin
I
Intervention
Incubation in ATP analog ATP gamma S or [35S]ATP gamma S in the presence of Ca2+
C
Comparator
Incubation in the absence of Ca2+, or exposure to ATP or [gamma-32P]ATP
O
Outcome
Activated tension and labeling of the 20,000-dalton myosin light chain (LC20)surrogate

The study provides mechanistic evidence supporting the myosin light chain kinase-phosphatase system in Ca2+ control of smooth muscle contraction.

Cite This Study

Cassidy et al. (1979) studied this question.

synapsesocial.com/papers/6a7027f653da1ffdb5079d83https://doi.org/10.1016/s0021-9258(19)86642-5
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characteristics of Ca2+- and Mg2+-induced tension development in chemically skinned smooth muscle fibers.1978 · 194 citations
  2. 2Contraction of detergent-treated smooth muscle.1978 · 80 citations
  3. 3Effect of phosphorylation of smooth muscle myosin on actin activation and Ca2+ regulation.1977 · 305 citations
  4. 4Ca2+ Dependent Phosphorylation of Bovine Aortic Actomyosin1978 · 58 citations
  5. 5A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity1964 · 1,880 citations