November 1, 2001Journal of Biological ChemistryOpen Access

A Mitogen-activated Protein Kinase-dependent Signaling Pathway in the Activation of Platelet Integrin αIIbβ3

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Population

Chinese hamster ovary (CHO) cells and platelets in a reconstituted integrin activation model

Design

Preclinical

Authors

ZLZhenyu Li XXXiaodong Xi XDXiaoping Du

Discussion

Member takes

Overview

Identifies PKG-MAPK as a platelet activation node; extends mechanistic insights but leaves open therapeutic targeting in thrombosis.

Structured PICO

Population

Chinese hamster ovary (CHO) cells and platelets in a reconstituted integrin activation model

Intervention

MEK inhibitors (PD98059 and U0126), dominant negative mutants of Raf-1 and MEK1, PKG inhibitors, and PKG activators

Outcome

Integrin alpha(IIb)beta(3) activation and platelet aggregationsurrogate

The study identifies a novel signaling pathway where von Willebrand factor binding to GPIb-IX activates PKG, which in turn stimulates the MAPK pathway to activate platelet integrin alpha(IIb)beta(3).

Cite This Study

Li et al. (2001) studied this question.

synapsesocial.com/papers/6a702ddfe71d69abee086e04 https://doi.org/10.1074/jbc.m106129200

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November 1, 2001Journal of Biological ChemistryOpen Access

A Mitogen-activated Protein Kinase-dependent Signaling Pathway in the Activation of Platelet Integrin αIIbβ3

View Full Paper

Ask AI

Bookmark

Population

Chinese hamster ovary (CHO) cells and platelets in a reconstituted integrin activation model

Design

Preclinical

Authors

ZLZhenyu Li XXXiaodong Xi XDXiaoping Du

Discussion

Member takes

Overview

Identifies PKG-MAPK as a platelet activation node; extends mechanistic insights but leaves open therapeutic targeting in thrombosis.

Structured PICO

Population

Chinese hamster ovary (CHO) cells and platelets in a reconstituted integrin activation model

Intervention

MEK inhibitors (PD98059 and U0126), dominant negative mutants of Raf-1 and MEK1, PKG inhibitors, and PKG activators

Outcome

Integrin alpha(IIb)beta(3) activation and platelet aggregationsurrogate

The study identifies a novel signaling pathway where von Willebrand factor binding to GPIb-IX activates PKG, which in turn stimulates the MAPK pathway to activate platelet integrin alpha(IIb)beta(3).

Cite This Study

Li et al. (2001) studied this question.

synapsesocial.com/papers/6a702ddfe71d69abee086e04 https://doi.org/10.1074/jbc.m106129200

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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