Why the study?
It was unclear whether ACTN3 protein expression levels and myosin heavy chain composition differ between ACTN3 R577X RR and RX genotypes in Japanese college-level male sprinters.
Are there differences in ACTN3 protein expression levels and myosin heavy chain composition between RR and RX genotypes in Japanese college-level male sprinters?
Are there differences in ACTN3 protein expression levels and myosin heavy chain composition between RR and RX genotypes in Japanese college-level male sprinters?
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RR genotype shows higher ACTN3 protein and IIx MyHC than RX in sprinters; leaves open performance implications.
Objective: Alpha-actinin (ACTN) 3 R577X polymorphisms have three genotypes: RR, RX and XX. Only RR and RX genotypes express ACTN3 protein in type II fibers. The purpose of this study was to clarify whether there are differences in ACTN3 protein expression levels and myosin heavy chain (MyHC) composition between RR and RX genotypes in Japanese college-level male sprinters. Materials and Methods: Forty-three Japanese college-level male sprinters participated in this study. Subjects were genotyped for ACTN3 R577X using a real-time polymerase chain reaction assay. Furthermore, muscle biopsies from the vastus lateralis muscle were obtained from a subset of subjects who had R allele and gave their consent (4 RR and 9 RX). ACTN3 protein expression levels were assessed by western blotting. MyHC composition was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis.
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Nakamura et al. (2019) studied this question.
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