January 1, 1996Muscle & Nerve

Porcine malignant hyperthermia: Genotype and contractile threshold of immature muscles

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Population

Muscles and cultured myotubes from neonatal piglets homozygous for Arg615 or for Cys615 ryanodine receptor…

Comparison

Exposure to varying potassium concentrations and… vs Comparison among normal, heterozygous, and MHS…

Design

Preclinical

Authors

EGEsther M. GallantAustralian National University RJR.C.K. JordanUniversity Health Network

Discussion

Member takes

Implication

Validates immature MHS piglet myotubes as a cellular MH model; leaves open translation to human susceptibility testing.

Structured PICO

Population

Muscles and cultured myotubes from neonatal piglets homozygous for Arg615 (normal) or for Cys615 (malignant hyperthermia-susceptible, MHS) ryanodine receptor alleles, and heterozygotes

Intervention

Exposure to varying potassium concentrations (K contractures) and square voltage pulses using whole cell patch-clamp techniques

Comparator

Comparison among normal (Arg615 homozygous), heterozygous, and MHS (Cys615 homozygous) genotypes

Outcome

Threshold for contraction (K+ concentration and voltage)surrogate

Immature muscles and myotubes from malignant hyperthermia-susceptible piglets express a significantly lower threshold for contraction, providing a suitable model for studying MH-related cellular abnormalities.

Cite This Study

Gallant et al. (1996) studied this question.

synapsesocial.com/papers/6a70443db27f15817827465a https://doi.org/10.1002/(sici)1097-4598(199601)19:1<68::aid-mus9>3.0.co;2-6

View Full Paper

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Porcine malignant hyperthermia: Genotype and contractile threshold of immature muscles

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Porcine malignant hyperthermia: Genotype and contractile threshold of immature muscles

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider