What question did this study set out to answer?

This research aims to investigate how intracellular Ca(2+) waves influence arrhythmogenic oscillatory depolarizations in cardiomyocytes.

July 18, 2008Circulation Research

Burst Emergence of Intracellular Ca²⁺Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na⁺–Ca²⁺Exchanger

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This research aims to investigate how intracellular Ca(2+) waves influence arrhythmogenic oscillatory depolarizations in cardiomyocytes.
Simultaneous fluorescence recording of intracellular Ca(2+) dynamics and membrane potential in rat hearts.
Used Langendorff-perfused rat hearts with fluo4/AM and RH237 under cytochalasin D perfusion.
Focused on low-K(+)-perfused conditions and application of isoproterenol to assess triggered activity.
Burst emergence of Ca(2+) waves led to arrhythmogenic oscillatory depolarizations in ventricular myocytes.
Oscillatory membrane potentials were abolished by ryanodine and Na(+)-Ca(2+) exchanger inhibitor SEA0400, confirming their critical role.
Low-K(+)-perfused hearts showed sporadic Ca(2+) waves without discernible membrane depolarization.

Structured PICO

Population

Langendorff-perfused rat hearts (ventricular myocytes on subepicardial surfaces)

Intervention

Rapid pacing and addition of isoproterenol to induce intracellular Ca2+ loading, followed by ryanodine or SEA0400 (Na+-Ca2+ exchanger inhibitor)

Outcome

Simultaneous fluorescence recording of intracellular Ca2+ ([Ca2+]i) dynamics and membrane potentials (Vm)surrogate

Demonstrates a mechanistic link between intracellular Ca2+ waves and arrhythmogenic oscillatory depolarizations via the Na+-Ca2+ exchanger in intact hearts.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

May inform NCX-targeted antiarrhythmics; hypothesis-generating in animal models, should not yet change practice.

Cite This Study

Fujiwara et al. (2008) studied this question.

synapsesocial.com/papers/6a709e42e36a167817e26f48 https://doi.org/10.1161/circresaha.108.176677

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Burst Emergence of Intracellular Ca²⁺Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na⁺–Ca²⁺Exchanger

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Burst Emergence of Intracellular Ca²⁺Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na⁺–Ca²⁺Exchanger

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider