What question did this study set out to answer?

To measure the second inward current (Isi) in isolated rat ventricular muscle cells and investigate its characteristics.

October 22, 1983Proceedings of the Royal Society B Biological Sciences

Characteristics of the second inward current in cells isolated from rat ventricular muscle

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Population

Single cells isolated from ventricular muscle of adult rat hearts

Comparison

Step depolarizations under voltage-clamp… vs Baseline/normal conditions

Design

Preclinical

Authors

MMMary R. MitchellUniversity of Aberdeen TPTheresa L. PowellThe Medical Center of Aurora DTDerek A. TerrarUniversity of Oxford

Discussion

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Implication

Hypothesis-generating for Ca-dependent currents in rodent myocytes; leaves open translation to human electrophysiology and in vivo temperature effects.

Key Points

To measure the second inward current (Isi) in isolated rat ventricular muscle cells and investigate its characteristics.
Cells were isolated from adult rat ventricular muscle and subjected to voltage-clamp measurements.
Changes in Isi were assessed under various ion substitutions, including the effects of Ca, Sr, and Ba on inactivation.
Optical monitoring was used to assess contraction timing related to Isi.
Isi was predominantly carried by Ca, reduced or abolished by several blockers (Mn, Ni, Cd, nifedipine).
A reduction in external Na decreased peak Isi when Ca was present, indicating Na is not a major charge carrier.
Kinetics of Isi were faster than previous multicellular studies, and contraction was observed shortly after peak Isi.

Structured PICO

Population

Single cells isolated from ventricular muscle of adult rat hearts

Intervention

Step depolarizations under voltage-clamp conditions with various ion substitutions (Mn, Ni, Cd, nifedipine, nimodipine, D600, Sr, Ba, reduced Na) and temperature changes

Comparator

Baseline/normal conditions

Outcome

Characteristics of the second inward current (Isi), including kinetics, peak amplitude, and relation to cellular contractionsurrogate

The study characterizes the calcium-dependent second inward current in adult rat ventricular cells, highlighting its temperature dependence and rapid coupling to cellular contraction.

Cite This Study

Mitchell et al. (1983) studied this question.

synapsesocial.com/papers/6a70a820c1a24a6142db8cd3 https://doi.org/10.1098/rspb.1983.0084

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Implication

Hypothesis-generating for Ca-dependent currents in rodent myocytes; leaves open translation to human electrophysiology and in vivo temperature effects.

Characteristics of the second inward current in cells isolated from rat ventricular muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Characteristics of the second inward current in cells isolated from rat ventricular muscle

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider