What question did this study set out to answer?

To determine how alternative splicing coordinates or uncouples the expression of the sarcomeric myosin gene MYH7b and its intronic microRNA, miR-499.

February 13, 2010Molecular and Cellular BiologyOpen Access

Uncoupling of Expression of an Intronic MicroRNA and Its Myosin Host Gene by Exon Skipping

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Authors

MBMatthew BellWest Virginia University MBMassimo BuvoliUniversity of Colorado Boulder LLLeslie A. LeinwandBoston University

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Implication

Mechanistic study reveals that alternative exon skipping disconnects miR-499 production from MYH7b protein synthesis, indicating independent regulatory control of intronic microRNAs.

Key Points

To determine how alternative splicing coordinates or uncouples the expression of the sarcomeric myosin gene MYH7b and its intronic microRNA, miR-499.
Analyzed the transcriptional activity and alternative splicing patterns of the MYH7b gene in striated muscle and brain tissues.
Evaluated the impact of exon skipping and premature termination codons on host protein translation and miR-499 biogenesis.
Assessed target regulation of the Sox6 transcription factor 3' untranslated region by endogenous miR-499 and its reciprocal effect on MYH7b transcription.
Exon skipping introduces a premature termination codon that selectively downregulates MYH7b protein synthesis without diminishing intronic miR-499 levels.
Endogenous miR-499 targets the 3' untranslated region of Sox6, repressing a key transcriptional inhibitor of MYH7b to dynamically balance gene expression.

Cite This Study

Bell et al. (2010) studied this question.

synapsesocial.com/papers/6a70ac4926a7f98052dd26fe https://doi.org/10.1128/mcb.01370-09

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Implication

Mechanistic study reveals that alternative exon skipping disconnects miR-499 production from MYH7b protein synthesis, indicating independent regulatory control of intronic microRNAs.

Uncoupling of Expression of an Intronic MicroRNA and Its Myosin Host Gene by Exon Skipping

Discussion

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Implication

Key Points

Cite This Study

Uncoupling of Expression of an Intronic MicroRNA and Its Myosin Host Gene by Exon Skipping

Discussion

Member takes

Implication

Key Points

Cite This Study

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