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January 1, 1953The Journal of Experimental MedicineOpen Access

The Intracellular Localization of Poliomyelitis Virus

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Population

Mice (infant and adult) infected with Type 2 poliomyelitis virus (MEF1 and Lansing strains)

Comparison

Cellular fractionation of central nervous system… vs Normal, uninoculated mice

Design

Preclinical

Authors

AKAlbert S. KaplanVanderbilt UniversityJMJoseph L. MelnickStatens Serum Institut

Discussion

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Implication

Animal data link nuclear viral accumulation to paralysis; leaves open translation to human polio mechanisms or therapies.

Structured PICO

P
Population
Mice (infant and adult) infected with Type 2 poliomyelitis virus (MEF1 and Lansing strains)
I
Intervention
Cellular fractionation of central nervous system tissue
C
Comparator
Normal, uninoculated mice
O
Outcome
Intracellular localization (cytoplasmic vs nuclear) of poliomyelitis virussurrogate

Type 2 poliomyelitis virus is initially found in the cytoplasm of infected mouse CNS cells and later in the nuclear fraction, with higher nuclear concentrations correlating with paralysis.

Limitations

  • The possibility has not been excluded however that the 'nuclear' sediment was contaminated by cytoplasmic particles too large to remain in the supernate.

Cite This Study

Kaplan et al. (1953) studied this question.

synapsesocial.com/papers/6a70bada87f921057127db34https://doi.org/10.1084/jem.97.1.91
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1ADAPTATION OF A LANSING STRAIN OF POLIOMYELITIS VIRUS TO NEWBORN MICE1951 · 19 citations
  2. 2INFLUENCE OF AGE ON SUSCEPTIBILITY AND ON IMMUNE RESPONSE OF MICE TO EASTERN EQUINE ENCEPHALOMYELITIS VIRUS1941 · 46 citations
  3. 3THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA1952 · 166 citations
  4. 4The Electron Microscopy of Fowl Pox Virus Within the Chorioallantoic Membrane1950 · 25 citations
  5. 5FREE AMINO ACIDS AND NUCLEIC ACID CONTENT OF CELL NUCLEI ISOLATED BY A MODIFICATION OF BEHRENS' TECHNIQUE1950 · 103 citations