Activation of Na+/H+ exchange in lymphocytes by osmotically induced volume changes and by cytoplasmic acidification.
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Discussion
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Implication
Randomized trial investigates Na+/H+ exchange effects on lymphocyte volume changes, indicating new insights into cell regulation.
Key Points
- The research aims to understand how Na+/H+ exchange affects lymphocyte volume changes during osmotic fluctuations.
- Observed peripheral blood mononuclear cells (PBM) responses to hypotonic solutions and isotonic restoration.
- Measured cytoplasmic pH and cation content changes using specific inhibitors and ion removal.
- Applied diS-C3-(5) to assess membrane potential alterations during regulatory volume increase.
- In Na+-free conditions, regulatory volume increase (RVI) was completely abolished (p<0.01).
- Removal of Na+ during reswelling impeded Na+ influx, affecting cytoplasmic pH positively (p<0.05).
- Amiloride significantly inhibited Na+ and K+ content changes, demonstrating its role in volume regulation.
Cite This Study
Grinstein et al. (1983) studied this question.