October 1, 1997Circulation Research
Angiotensin II Stimulates Tyrosine Phosphorylation of Phospholipase C-γ–Associated Proteins
Population
Rat aortic smooth muscle cells, mouse aortic smooth muscle cells, and NIH-3T3 cells
Comparison
Angiotensin II (100 nmol/L for 5 minutes) vs Unstimulated cells, wild-type cells, or cells…
Design
Preclinical
Authors
Discussion
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Implication
Hypothesis-generating for Src in Ang II vascular signaling; leaves open relevance to hypertension or atherosclerosis.
Structured PICO
P
Population
Rat aortic smooth muscle (RASM) cells, mouse aortic smooth muscle (MASM) cells (wild-type and c-Src knockout), and NIH-3T3 cells (wild-type and v-Src-transformed)
I
Intervention
Angiotensin II (100 nmol/L for 5 minutes)
C
Comparator
Unstimulated cells, wild-type cells, or cells treated with Src-family kinase inhibitor CP-118,556
O
Outcome
Tyrosine phosphorylation of PLC-gamma-associated proteins (specifically p97)surrogate
Identifies a 97-kD PLC-gamma-associated protein whose Src-dependent tyrosine phosphorylation is a key event in Angiotensin II signal transduction in vascular smooth muscle cells.
Cite This Study
Schmitz et al. (1997) studied this question.