July 1, 1998Journal of Biological ChemistryOpen Access

Temporal Dispersion of Activation of Phospholipase C-β1 and -γ Isoforms by Angiotensin II in Vascular Smooth Muscle Cells

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Population

Cultured rat vascular smooth muscle cells (VSMCs)

Design

Preclinical

Authors

MUMasuko Ushio‐FukaiVascular / Pulmonary Vascular KGKathy K. GriendlingVascular Medicine MAMarjorie AkersEmory University

Discussion

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Implication

AT1 receptor signaling shows temporal PLC isoform switching; leaves open translation to human vascular disease or therapies.

Structured PICO

Population

Cultured rat vascular smooth muscle cells (VSMCs)

Intervention

Angiotensin II stimulation with electroporation of specific antibodies (anti-PLC-beta1, anti-Galphaq/11, anti-Galpha12, anti-PLC-gamma) or tyrosine kinase inhibitor (genistein)

Outcome

Inositol trisphosphate (IP3) formation and PLC activity at 15 seconds and 30 secondssurrogate

AT1 receptors in vascular smooth muscle cells sequentially couple to PLC-beta1 via heterotrimeric G proteins and to PLC-gamma via a downstream tyrosine kinase, demonstrating a temporally controlled signaling network.

Cite This Study

Ushio‐Fukai et al. (1998) studied this question.

synapsesocial.com/papers/6a715bac35aa2c282ce2a8c0 https://doi.org/10.1074/jbc.273.31.19772

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Temporal Dispersion of Activation of Phospholipase C-β1 and -γ Isoforms by Angiotensin II in Vascular Smooth Muscle Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Temporal Dispersion of Activation of Phospholipase C-β1 and -γ Isoforms by Angiotensin II in Vascular Smooth Muscle Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider