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August 15, 2005FEBS LettersOpen Access

Activation of Epac1 with 007 decreased endothelial cell monolayer permeability and induced actin cytoskeleton reorganization; these effects were dependent on VE-cadherin and inhibited by Epac1 siRNA.

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Population

Human umbilical vein endothelial cells, including VE-cadherin knock-out cells and cells re-expressing…

Design

Preclinical

Authors

MKMatthijs R.H. KooistraMCMonica CoradaEDElisabetta Dejana

Discussion

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Overview

Epac1 may modulate endothelial barrier function in vitro; leaves open therapeutic targeting in vascular leak syndromes.

Structured PICO

P
Population
Human umbilical vein endothelial cells (HUVECs), including VE-cadherin knock-out cells and cells re-expressing VE-cadherin
I
Intervention
Activation of Epac1 with cAMP analog 8CPT-2'O-Me-cAMP (007) and inhibition with siRNA against Epac1
O
Outcome
Endothelial cell monolayer permeability and cell-cell junction formationsurrogate

Epac1 controls VE-cadherin-mediated cell junction formation and induces reorganization of the actin cytoskeleton in human endothelial cells.

Cite This Study

Kooistra et al. (2005) studied this question.

synapsesocial.com/papers/6a70ea5ba7fbea1e440840f5https://doi.org/10.1016/j.febslet.2005.07.080
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Epac1 Is Crucial for Maintenance of Endothelial Barrier Function through A Mechanism Partly Independent of Rac12020 · 18 citations
  2. 2Cyclic AMP Phosphodiesterase 4D (PDE4D) Tethers EPAC1 in a Vascular Endothelial Cadherin (VE-Cad)-based Signaling Complex and Controls cAMP-mediated Vascular Permeability2010 · 95 citations
  3. 3cAMP signalling in the vasculature: the role of Epac (exchange protein directly activated by cAMP)2014 · 69 citations
  4. 4Activation of Epac/Rap1 attenuates venular permeability response to platelet activating factor (PAF) in vivo2006
  5. 5Selective EPAC1 Activators Enhance Endothelial Function in Models of Vascular Inflammation2025 · 5 citations