July 1, 1991Journal of Biological ChemistryOpen Access

Differential effect of pH on thromboxane A2/prostaglandin H2 receptor agonist and antagonist binding in human platelets

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Population

Washed human platelets and solubilized platelet membranes

Comparison

Changes in pH and treatment with… vs Physiological pH (7.4)

Design

Preclinical

Authors

PMPhilip R. MayeuxUniversity of Arkansas for Medical Sciences TMThomas A. MorinelliAllen University TWT.C. WilliamsMedical University of South Carolina

Discussion

Member takes

Implication

pH effects on TXA2/PGH2 binding should not alter clinical platelet assays; leaves open histidine's role in receptor regulation in vivo.

Structured PICO

Population

Washed human platelets and solubilized platelet membranes

Intervention

Changes in pH (pH 4-6, 5.0, 6.0) and treatment with histidine-modifying reagents (diethyl pyrocarbonate and N-bromosuccinimide)

Comparator

Physiological pH (7.4)

Outcome

Binding affinity (Kd) and number of receptors (Bmax) for TXA2/PGH2 receptor agonists and antagonistssurrogate

Agonist and antagonist binding characteristics differ for the TXA2/PGH2 receptor, with lower pH increasing agonist affinity, suggesting histidine residues play an important role in ligand binding.

Cite This Study

Mayeux et al. (1991) studied this question.

synapsesocial.com/papers/6a70f24075498292b70a7017 https://doi.org/10.1016/s0021-9258(18)92764-x

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