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June 1, 1983Journal of Lipid ResearchOpen Access

Lipoprotein lipase-catalyzed hydrolysis of phosphatidylcholine of guinea pig very low density lipoproteins and discoidal complexes of phospholipid and apolipoprotein: effect of apolipoprotein C-II on the catalytic mechanism

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Population

Discoidal phospholipid complexes prepared with apoC-III and di[C]palmitoyl phosphatidylcholine, and guinea…

Comparison

Presence of apolipoprotein C-II (apoC-II) vs Absence of apolipoprotein C-II

Design

Preclinical

Authors

KSKeiko ShiraiUniversidad Autónoma MetropolitanaTFT J FitzharrisUniversity of Cincinnati Medical CenterMSMasaki ShinomiyaYamagata University

Discussion

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Implication

Should not change clinical lipid management; leaves open ApoC-II as LpL regulator pending human validation.

Structured PICO

P
Population
Discoidal phospholipid complexes prepared with apoC-III and di[(14)C]palmitoyl phosphatidylcholine (DPPC), and guinea pig very low density lipoproteins (VLDL(p))
I
Intervention
Presence of apolipoprotein C-II (apoC-II)
C
Comparator
Absence of apolipoprotein C-II (apoC-III complex containing no apoC-II)
O
Outcome
Rate of DPPC and trioleoylglycerol hydrolysis catalyzed by purified bovine milk lipoprotein lipase (LpL), measured by enzyme kinetic parameters (Vmax and Km)surrogate

Apolipoprotein C-II enhances phospholipid hydrolysis by lipoprotein lipase primarily by increasing the maximal velocity (Vmax) for the substrates, while decreasing the apparent Km for triacylglycerol hydrolysis.

Cite This Study

Shirai et al. (1983) studied this question.

synapsesocial.com/papers/6a71730331a3df824329a2c8https://doi.org/10.1016/s0022-2275(20)37947-5
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Hydrolysis of guinea pig nascent very low density lipoproteins catalyzed by lipoprotein lipase: activation by hjman apolipoprotein C-II.1981 · 37 citations
  2. 2Lipoprotein Lipase: Some Effects of Activator Proteins1980 · 127 citations
  3. 3Radioimmunoassay of Human Apolipoprotein CII1977 · 142 citations
  4. 4A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION1959 · 49,113 citations