Key result
Silencing RyR3 expression in zebrafish embryos reduced the PJF/JF ratio from 0.79 to 0.03 and Ca(2+) spark frequency from 0.083 to 0.001 sarcomere(-1) s(-1) compared with wild-type.
Population
Zebrafish (Danio rerio) one-cell stage embryos and larvae at 72 h postfertilization
Comparison
Injection with a morpholino designed to… vs Wild-type (WT) zebrafish
Design
Preclinical
Follow-up
72 h postfertilization
Authors
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RyR3 may mediate Ca sparks in fish muscle; hypothesis-generating for its role in mammalian cardiac Ca handling.
Absolute Event Rate: 0.03% vs 0.79%
This study provides evidence that RyR3 is the main constituent of parajunctional feet and the primary contributor to the sarcoplasmic reticulum Ca(2+) flux underlying Ca(2+) sparks in fully differentiated fish fibers.
Perni et al. (2015) studied this question. Morpholino silencing of RyR3 expression vs. Wild-type (WT) was evaluated on PJF/JF ratio. Silencing RyR3 expression in zebrafish embryos reduced the PJF/JF ratio from 0.79 to 0.03 and Ca(2+) spark frequency from 0.083 to 0.001 sarcomere(-1) s(-1) compared with wild-type.
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