Key result
Expression of RyR3 in adult mouse skeletal muscle fibres produced repetitive spontaneous transient elevations of intracellular Ca2+ in approximately 90% of fibres, unlike RyR1 expression.
Population
Adult mouse skeletal muscle fibres
Comparison
In vivo expression of RyR3 using a GFP-RyR3 DNA… vs Muscle fibres expressing a GFP-RyR1 construct
Design
Preclinical
Authors
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RyR3 may drive spontaneous Ca2+ release in skeletal muscle; leaves open its isoform-specific role in human myopathies or excitation-contraction coupling.
Absolute Event Rate: 90% vs 0%
RyR3 acts as a potent source of voltage-independent Ca2+ release activity in adult mouse skeletal muscle fibres, but does not appear to contribute to discrete voltage-activated Ca2+ release events.
Legrand et al. (2007) studied this question. GFP-RyR3 DNA construct vs. GFP-RyR1 construct was evaluated on Repetitive spontaneous transient elevations of intracellular Ca2+. Expression of RyR3 in adult mouse skeletal muscle fibres produced repetitive spontaneous transient elevations of intracellular Ca2+ in approximately 90% of fibres, unlike RyR1 expression.
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