What question did this study set out to answer?

This research investigates the role of P2Y12 and P2Y1 receptors in platelet aggregation under flow conditions.

December 1, 2001Blood

Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow

View Full Paper

Ask AI

Bookmark

Population

Heparinized whole blood

Comparison

Blockade of ADP receptors P2Y12 and P2Y1, alone… vs Control samples

Design

Preclinical

Authors

NTNancy A. TurnerRice University JMJoel L. MoakeRice University LMLarry V. McIntireFreeman Health System

Discussion

Member takes

Implication

Dual P2Y12/P2Y1 blockade may be needed to inhibit high-shear thrombus formation; leaves open clinical translation from animal models.

Key Points

This research investigates the role of P2Y12 and P2Y1 receptors in platelet aggregation under flow conditions.
Heparinized whole blood tested under shear rates of 750, 1500, and 3000 s(-1) for 30 seconds.
Platelet aggregation measured using flow cytometry and epifluorescence video microscopy.
Combination of P2Y12 antagonist ARMX and P2Y1 antagonist A3P5P evaluated for efficacy.
ARKX reduced shear-induced aggregation by 56%, 54%, and 16% at shear rates of 750, 1500, and 3000 s(-1), respectively.
A3P5P inhibited shear-induced aggregation by 40%, 30%, and 29%, respectively.
Blocking both P2Y12 and P2Y1 reduced platelet deposition by 72% compared to control.

Structured PICO

Population

Heparinized whole blood

Intervention

Blockade of ADP receptors P2Y12 (with AR-C69 931MX) and P2Y1 (with A3P5P), alone or in combination

Comparator

Control samples

Outcome

Extent of shear-induced platelet aggregation and platelet deposition on vWf-collagensurrogate

Combined blockade of P2Y12 and P2Y1 receptors is required to effectively inhibit platelet aggregation and thrombus formation under high shear flow conditions.

Cite This Study

Turner et al. (2001) studied this question.

synapsesocial.com/papers/6a71f6575d37378ac1deee5e https://doi.org/10.1182/blood.v98.12.3340

Topics

Dual antiplatelet therapy

View Full Paper

Ask AI

Bookmark

Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider