Feedback regulation of angiotensin converting enzyme activity and mRNA levels by angiotensin II.
Why the study?
Does intravenous Angiotensin II infusion regulate ACE mRNA expression and enzymatic activity in male Sprague-Dawley rats?
Population
Male Sprague-Dawley rats (n=32 total, 4 groups of n=8)
Comparison
Intravenous Angiotensin II infusion for 3 days… vs Vehicle infusion (control)
Design
Preclinical
Follow-up
3 days
Authors
Discussion
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Member takes
Implication
Provides mechanistic insight into ACE regulation in rat models; leaves open human translation and clinical relevance.
Structured PICO
Does intravenous Angiotensin II infusion regulate ACE mRNA expression and enzymatic activity in male Sprague-Dawley rats?
P
Population
Male Sprague-Dawley rats (n=32 total, 4 groups of n=8)
I
Intervention
Intravenous Angiotensin II infusion for 3 days at 100 (low dose), 300 (medium dose), or 1,000 (high dose) ng/kg per minute
C
Comparator
Vehicle infusion (control)
O
Outcome
ACE mRNA levels in the lung and testis, and ACE enzymatic activity in lung, serum, and testissurrogate
Angiotensin II exerts negative feedback regulation on ACE gene expression and enzymatic activity in major sites of ACE synthesis.
Cite This Study
Schunkert et al. (1993) studied this question.