Regulation of glucose uptake by muscle. 3. The effects of insulin, anoxia, salicylate and 2:4-dinitrophenol on membrane transport and intracellular phosphorylation of glucose in the isolated rat heart

In previous papers (Randle & Smith, 1958a, b) evidence was presented that insulin, anoxia and substances such as salicylate or 2:4-dinitrophenol, which inhibit oxidative phosphorylation, increase the uptake of glucose and D-xylose by isolated rat diaphragm by accelerating the transfer of these sugars across the muscle-cell membrane. The con- clusion was drawn that the transfer process for sugars in muscle is inhibited by a substance generated during oxidative phosphorylation and that insulin activates the transfer process by inter- fering with the action of this substance.