What question did this study set out to answer?

To determine the tissue expression and anatomical localization of the IGF-binding protein BP-3A in the fetal and adult rat central nervous system.

October 1, 1989Molecular EndocrinologyOpen Access

The Fetal Rat Binding Protein for Insulin-Like Growth Factors is Expressed in the Choroid Plexus and Cerebrospinal Fluid of Adult Rats

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Authors

LTLucy Y.-H. TsengNational Institutes of Health ABAlexandra L. BrownUniversity of California, Santa Barbara YYYvonne W.-H. YangUnited States Department of Veterans Affairs

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Implication

Preclinical study reveals localization of IGF-binding protein BP-3A to the choroid plexus and cerebrospinal fluid in adult rats, suggesting a role in transporting IGF-II across the brain.

Key Points

To determine the tissue expression and anatomical localization of the IGF-binding protein BP-3A in the fetal and adult rat central nervous system.
Measured BP-3A mRNA levels in brain stem, cerebral cortex, and hypothalamus tissues from 21-day gestation and adult rats using Northern blot analysis.
Mapped BP-3A mRNA synthesis across coronal adult rat brain sections using in situ hybridization with 35S-labeled 48-base oligonucleotides.
Analyzed adult rat cerebrospinal fluid with specific antibodies and binding assays to identify BP-3A protein and assess its affinity for IGF-I and IGF-II.
BP-3A mRNA was detected at high levels in the fetal brain stem, cerebral cortex, and hypothalamus, with expression persisting into adulthood.
In situ hybridization localized BP-3A mRNA synthesis exclusively to the choroid plexus spanning the medial preoptic nucleus to the arcuate nucleus.
A 30 kDa protein recognized by BP-3A antibodies was identified in cerebrospinal fluid, demonstrating equal binding affinity for both IGF-I and IGF-II.

Cite This Study

Tseng et al. (1989) studied this question.

synapsesocial.com/papers/6a722a29febe604dd70a7757 https://doi.org/10.1210/mend-3-10-1559

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Implication

Preclinical study reveals localization of IGF-binding protein BP-3A to the choroid plexus and cerebrospinal fluid in adult rats, suggesting a role in transporting IGF-II across the brain.

The Fetal Rat Binding Protein for Insulin-Like Growth Factors is Expressed in the Choroid Plexus and Cerebrospinal Fluid of Adult Rats

Discussion

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Implication

Key Points

Cite This Study

The Fetal Rat Binding Protein for Insulin-Like Growth Factors is Expressed in the Choroid Plexus and Cerebrospinal Fluid of Adult Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

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