What question did this study set out to answer?

To evaluate the sarcolemmal Na+-K+-ATPase activity and its relationship with diabetes in rats.

September 1, 1983AJP Cell Physiology

Sarcolemmal Na+-K+-ATPase activity in diabetic rat heart

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Population

Rats with chronic diabetes induced by a streptozotocin (65 mg/kg, iv) injection

Comparison

Streptozotocin-induced diabetes vs Control preparations (non-diabetic rats)

Design

Preclinical

Authors

GPGrant N. PierceGeneral / Preventive / Lipids NDNaranjan S. DhallaElectrophysiology

Discussion

Member takes

Implication

Preclinical insulin reversal of depressed Na+-K+-ATPase in diabetic rat hearts leaves open translation to human diabetic cardiomyopathy.

Key Points

To evaluate the sarcolemmal Na+-K+-ATPase activity and its relationship with diabetes in rats.
Chronic diabetes induced in rats via streptozotocin injection (65 mg/kg, iv).
Heart sarcolemmal membranes isolated through hypotonic shock-LiBr treatment and analyzed for enzymatic activities.
Comparative evaluation of enzymatic activities and membrane composition between diabetic and control preparations.
Elevated Mg2+-dependent ATPase activity in diabetic rats; reduced 5'-nucleotidase and K+-p-nitrophenylphosphatase activities.
Depressed latent Na+-K+-ATPase activities in the diabetic heart; normalized after insulin treatment.
Increased cholesterol content in diabetic sarcolemmal membranes, affecting enzyme resistance to treatments.

Structured PICO

Population

Rats with chronic diabetes induced by a streptozotocin (65 mg/kg, iv) injection

Intervention

Streptozotocin-induced diabetes (with a subgroup receiving insulin treatment)

Comparator

Control preparations (non-diabetic rats)

Outcome

Sarcolemmal Na+-K+-ATPase activitysurrogate

Chronic experimental diabetes in rats leads to defects in sarcolemmal enzymatic activities and composition, including depressed latent Na+-K+-ATPase activity, which is reversible with insulin.

Cite This Study

Pierce et al. (1983) studied this question.

synapsesocial.com/papers/6a723930a7fbea1e44089eec https://doi.org/10.1152/ajpcell.1983.245.3.c241

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Sarcolemmal Na+-K+-ATPase activity in diabetic rat heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Sarcolemmal Na+-K+-ATPase activity in diabetic rat heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider