A rapid method for determination of endoproteinase substrate specificity: specificity of the 3C proteinase from hepatitis A virus.

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Population

3C proteinase from hepatitis A virus (HAV-3C) and peptide substrates

Design

Preclinical

Authors

Discussion

HAV-3C P'1 preferences defined; leaves open translation to antiviral design pending cellular and in vivo validation.

Population

3C proteinase from hepatitis A virus (HAV-3C) and peptide substrates

Intervention

Rapid screening method using mixtures of N-terminal acetylated peptides and Edman degradation

Outcome

Preferred amino acid residues at the P'1 and P'2 positions of peptide substratessurrogate

A rapid screening method using Edman degradation successfully determined the substrate specificity of the HAV-3C proteinase.

Petithory et al. (1991) studied this question.

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