What question did this study set out to answer?

To identify the origins of tumorigenicity in BHK 21/13 cells and determine whether polyoma virus induces transformation directly or selects for pre-existing variants.

September 1, 1968International Journal of Cancer

The basis of the tumorigenicity of BHK 21 cells

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Authors

OJOswald JarrettVeterinary Medicines Directorate IMIan MacphersonThe Wistar Institute

Discussion

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Overview

Experimental study reveals selection of rare tumorigenic variants during serial passage of BHK 21 cells, suggesting viral transformation directly drives tumor formation.

Key Points

To identify the origins of tumorigenicity in BHK 21/13 cells and determine whether polyoma virus induces transformation directly or selects for pre-existing variants.
Assessed transplantability and variant cell frequency in BHK 21/13 cultures 50 generations after single-cell cloning.
Recloned stocks to isolate non-tumorigenic cell lines and tracked variant frequency over prolonged in vitro passage.
Evaluated the tumorigenicity of polyoma-transformed derivatives compared to uninfected parental cells.
Early-passage BHK 21/13 stocks contained approximately 0.001% tumorigenic variant cells, which increased to over 90% during continued in vitro culture.
Non-tumorigenic populations were successfully isolated through recloning, confirming cellular heterogeneity in cultured stocks.
Polyoma virus transformation generated highly tumorigenic cells via direct virus-cell interaction rather than selective enrichment of pre-existing variants.

Cite This Study

Jarrett et al. (1968) studied this question.

synapsesocial.com/papers/6a72c21235aa2c282ce36b48 https://doi.org/10.1002/ijc.2910030514

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September 1, 1968International Journal of Cancer

The basis of the tumorigenicity of BHK 21 cells

View Full Paper

Ask AI

Bookmark

Authors

OJOswald JarrettVeterinary Medicines Directorate IMIan MacphersonThe Wistar Institute

Discussion

Member takes

Overview

Experimental study reveals selection of rare tumorigenic variants during serial passage of BHK 21 cells, suggesting viral transformation directly drives tumor formation.

Key Points

To identify the origins of tumorigenicity in BHK 21/13 cells and determine whether polyoma virus induces transformation directly or selects for pre-existing variants.
Assessed transplantability and variant cell frequency in BHK 21/13 cultures 50 generations after single-cell cloning.
Recloned stocks to isolate non-tumorigenic cell lines and tracked variant frequency over prolonged in vitro passage.
Evaluated the tumorigenicity of polyoma-transformed derivatives compared to uninfected parental cells.
Early-passage BHK 21/13 stocks contained approximately 0.001% tumorigenic variant cells, which increased to over 90% during continued in vitro culture.
Non-tumorigenic populations were successfully isolated through recloning, confirming cellular heterogeneity in cultured stocks.
Polyoma virus transformation generated highly tumorigenic cells via direct virus-cell interaction rather than selective enrichment of pre-existing variants.

Cite This Study

Jarrett et al. (1968) studied this question.

synapsesocial.com/papers/6a72c21235aa2c282ce36b48 https://doi.org/10.1002/ijc.2910030514

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