Key result
Mutation of the PARP-I binding site adjacent to the distal MCAT element in the chicken cTnT promoter produced a non-cell-specific promoter in vitro and in zebrafish embryos.
Population
Zebrafish embryos and in vitro cell cultures (cardiomyocyte, myoblast, and fibroblast)
Comparison
Injection of chicken cardiac troponin T promoter… vs Wild-type/unmutated constructs
Design
Preclinical
Authors
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Supports evolutionary conservation of PARP-I promoter specificity across chordates; leaves open applicability to human cTnT regulation.
The PARP-I transcriptional regulatory mechanism governing muscle specificity of the chicken cTnT promoter is conserved across chordate classes spanning at least 350 million years of evolution.
Tidyman et al. (2003) studied this question. cTnT promoter constructs was evaluated on GFP expression and luciferase activity. Mutation of the PARP-I binding site adjacent to the distal MCAT element in the chicken cTnT promoter produced a non-cell-specific promoter in vitro and in zebrafish embryos.
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