What question did this study set out to answer?

To identify the major calcium current components in chick ventricle cells and their characteristics.

May 1, 1989AJP Heart and Circulatory Physiology

Low-threshold current is major calcium current in chick ventricle cells

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Population

Single myocytes dissociated from the apical ventricles of 14-day-old chick embryos, maintained in culture…

Design

Preclinical

Authors

SKSeiko KawanoKurume University RDRemy DehaanCharles Sturt University

Discussion

Member takes

Implication

Does not support clinical practice changes; leaves open T-type current roles in mammalian cardiac development.

Key Points

To identify the major calcium current components in chick ventricle cells and their characteristics.
Dissociated single myocytes from 14-day-old chick embryos were cultured for 24 hours.
Whole cell patch-clamp analysis was performed to measure calcium current (ICa) components.
T-type and L-type currents were differentiated based on voltage thresholds and responsiveness to specific blockers.
T-type current (IT) had a greater current density than reported in adult cardiac tissue.
L-type current (IL) was activated at -40 mV and blocked by D 600.
IT was identified as the major contributor to ICa, larger than IL.

Structured PICO

Population

Single myocytes dissociated from the apical ventricles of 14-day-old chick embryos, maintained in culture for 24 h

Intervention

Whole cell patch-clamp analysis with pharmacological blockade (1 microM D 600 or 120 microM Ni2+)

Outcome

Characterization of T-type (IT) and L-type (IL) calcium currentssurrogate

In 14-day-old chick embryo ventricular myocytes, the T-type calcium current is the major contributor to total calcium current, contrasting with adult cardiac tissue.

Cite This Study

Kawano et al. (1989) studied this question.

synapsesocial.com/papers/6a73410a2ac1135ea9c8f9bf https://doi.org/10.1152/ajpheart.1989.256.5.h1505

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Low-threshold current is major calcium current in chick ventricle cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Low-threshold current is major calcium current in chick ventricle cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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