What question did this study set out to answer?

To examine calcium currents in isolated neonatal rat ventricular myocytes and characterize their modulation by various agents.

October 1, 1987The Journal of PhysiologyOpen Access

Calcium current in isolated neonatal rat ventricular myocytes.

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Population

Isolated neonatal rat ventricular heart muscle cells grown in primary culture

Comparison

Calcium channel antagonist D600 and calcium… vs Baseline/control conditions

Design

Preclinical

Authors

NCNeri M. CohenGreater Baltimore Medical Center WLW. Jonathan LedererElectrophysiology

Discussion

Member takes

Implication

No immediate clinical translation from neonatal rat data; leaves open applicability to adult human L-type Ca channel modulation.

Key Points

To examine calcium currents in isolated neonatal rat ventricular myocytes and characterize their modulation by various agents.
Isolated neonatal rat ventricular myocytes were cultured and analyzed using the whole-cell voltage-clamp technique.
Voltage protocols focused on the L type calcium channels to measure transient and steady-state ICa components.
D600 and BAY K8644 were used to assess modulation effects on ICa conductance and inactivation parameters.
D600 reduced ICa conductance and shifted d infinity to more positive potentials without affecting inactivation rate (p not stated).
BAY K8644 shifted both d infinity and f infinity to more negative potentials, increasing inactivation rate at +5 to +55 mV (p not stated).
A significant steady-state component of ICa was observed at plateau potentials, consistent with window current analysis.

Structured PICO

Population

Isolated neonatal rat ventricular heart muscle cells grown in primary culture

Intervention

Calcium channel antagonist D600 and calcium channel modulator BAY K8644

Comparator

Baseline/control conditions

Outcome

Transient and steady-state components of L-type calcium current (ICa), including steady-state activation (d infinity) and inactivation (f infinity) parameterssurrogate

The study characterizes the steady-state and transient components of L-type calcium currents in neonatal rat ventricular myocytes and demonstrates how they are modulated by specific calcium channel agents.

Cite This Study

Cohen et al. (1987) studied this question.

synapsesocial.com/papers/6a762f11b52b7ee7dcfbe4cd https://doi.org/10.1113/jphysiol.1987.sp016732

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Calcium current in isolated neonatal rat ventricular myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Calcium current in isolated neonatal rat ventricular myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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