What question did this study set out to answer?

To determine whether tissue-specific differences in vascular endothelial growth factor and its receptor expression explain why diabetes causes myocardial vascular deficits alongside retinal neovascularization.

January 22, 2002CirculationOpen Access

Decreased Cardiac Expression of Vascular Endothelial Growth Factor and Its Receptors in Insulin-Resistant and Diabetic States

View Full Paper

Ask AI

Bookmark

Population

Diabetic and insulin-resistant nondiabetic rats, human ventricles from diabetic patients and nondiabetic…

Comparison

Insulin treatment vs Untreated diabetic/insulin-resistant state or…

Design

Preclinical

Authors

ECEva ChouWalter Reed National Military Medical Center ISIzumi SuzumaKyoto University KWKerrie J. WayThe University of Melbourne

Discussion

Member takes

Implication

Suggests tissue-specific VEGF dysregulation may underlie diabetic cardiac complications; hypothesis-generating and requires clinical validation.

Key Points

To determine whether tissue-specific differences in vascular endothelial growth factor and its receptor expression explain why diabetes causes myocardial vascular deficits alongside retinal neovascularization.
Measured mRNA and protein expression of VEGF, VEGF-R1, and VEGF-R2 across myocardium, retina, glomeruli, and aorta in diabetic and insulin-resistant nondiabetic rats, with and without insulin therapy.
Assessed VEGF and VEGF-R2 expression in ventricular tissue from human diabetic and nondiabetic donors, and evaluated VEGF mRNA expression in cultured rat neonatal cardiomyocytes exposed to insulin.
Myocardial VEGF, VEGF-R1, and VEGF-R2 expression fell by 40% to 70% in diabetic and insulin-resistant rats, matching a 2-fold reduction of VEGF and VEGF-R2 in human diabetic ventricles.
Retinal and glomerular expression of VEGF and its receptors increased 2-fold in diabetic and insulin-resistant rats, but systemic insulin therapy completely normalized levels across all tissues.

Structured PICO

Population

Diabetic and insulin-resistant nondiabetic rats, human ventricles from diabetic patients and nondiabetic donors, and cultured rat neonatal cardiac myocytes

Intervention

Insulin treatment

Comparator

Untreated diabetic/insulin-resistant state or nondiabetic controls

Outcome

Expression of mRNA and protein for VEGF and its receptors (VEGF-R1 and VEGF-R2)surrogate

Differential regulation of VEGF in cardiac versus microvascular tissues in diabetes provides a mechanism for poor myocardial collateral formation alongside retinal neovascularization, which can be normalized by insulin.

Cite This Study

Chou et al. (2002) studied this question.

synapsesocial.com/papers/6a737ce130bc69be694e61e4 https://doi.org/10.1161/hc0302.102143

View Full Paper

Ask AI

Bookmark

Implication

Suggests tissue-specific VEGF dysregulation may underlie diabetic cardiac complications; hypothesis-generating and requires clinical validation.

Decreased Cardiac Expression of Vascular Endothelial Growth Factor and Its Receptors in Insulin-Resistant and Diabetic States

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Decreased Cardiac Expression of Vascular Endothelial Growth Factor and Its Receptors in Insulin-Resistant and Diabetic States

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider